12/20/2023 0 Comments Palindromic sequence zinc finger![]() ![]() (C) (Top) TALE protein in complex with target DNA (grey) (PDB ID: 3UGM). Zinc-finger proteins can be designed to recognize unique ‘left’ and ‘right’ half-sites. We propose that GATApal is important for positive regulation of GATA-1 expression in erythroid cells. ZFN target sites consist of two zinc-finger binding sites separated by a 57-bp spacer sequence recognized by the Fok I cleavage domain. Deletion of the partial motif changes the site to a normal GATA site and also reduces by as much as eightfold the activity of the GATA-1 promoter in an erythroid precursor cell. Zinc-finger nucleases ( ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Two monomers of yeast transcription factor ADR1 bind a palindromic sequence. ![]() GATApal is a palindromic site composed of one complete and one partial (GAT) canonical motif. The PAR shares sequence homology with the N-terminal regions of other zinc finger. The site occurs in the GATA-1 gene promoters of chickens, mice, and humans but occurs very infrequently in other vertebrate genes known to be regulated by GATA proteins. Here we identify a DNA sequence (GATApal) for which the GATA-1 amino-terminal finger makes a critical contribution to the strength of binding. We detected zinc-dependent binding of a zinc-induced protein to a specific sequence, the zinc transcriptional regulatory element (ZTRE), in the SLC30A5 (zinc. The carboxy-terminal finger is necessary and sufficient for specific binding to the consensus GATA recognition sequence: mutant proteins containing only the amino-terminal finger do not bind. Use this guide to learn more about the genome engineering technologies and find the plasmids that are available from Addgenes depositing scientists. GATA-1, a transcription factor essential for the development of the erythroid lineage, contains two adjacent highly conserved zinc finger motifs. Addgenes plasmid repository contains a variety of tools to target and edit genomes. ![]()
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